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KMID : 0665420090240010084
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Korean Journal of Food Culture
2009 Volume.24 No. 1 p.84 ~ p.89
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Studies on Potato Glycoalkaloid Determination by Acid-hydrolysis Method
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Yoon Kyung-Soon
Byun Gwang-In
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Abstract
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This paper was conducted to evaluate aglycones and carbohydrates produced by acid hydrolysis of three potato glycoalkaloids [(PGA); -chaconine, -solanine, and demissine] in potatoes. Standard solanidine and demissidine were dissolved in 1N HCl and then heated at for 10-120 min. Solanidine was rapidly decomposed during acid hydrolysis and one peak that was identified as solantherene (=379) by GC-MS was detected. The transformation solanidine to solanthrene was approximately 50% complete after 10 min, approximately 90% complete after 60 min and 100% complete after 120 min. Demissidine was hydrolyzed using the same method that was used to hydrolyze the solanidine. However, demissidine produced only one peak upon GC-MS (=399) analysis and was found to be very stable at increased temperatures. Acidy hydrolysis of -chaconine, -solanine and demissine resulted in the decomposition of -chaconine and -solanine to solanidine and solanthrene, respectively. Therefore, this hydrolysis method should not be utilized to produce PGA combining with solanidine as aglycone. The individual carbohydrates produced by the two PGAs by hydrolysis were very stable at increased temperatures; therefore, it was possible to quantify these PGAs based on calculation of the individual carbohydrate content. Conversely, because demissidine produced by the hydrolysis of demissine was extremely stable at increased temperatures, it was possible to quantify the PGA based on the aglycone produced by hydrolysis.
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KEYWORD
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potato, glycoalkaloid, solanidine, demissidine, gas chromatography, liquid chromatography-mass spectrometry, aglycon
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